Scanning microscopic method having high axial resolution

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United States of America Patent

PATENT NO 7202953
SERIAL NO

09868845

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The invention relates to a method for optically detecting at least one entity which is arranged on a substrate. The at least one entity is scanned with a measuring volume using at least one radiation source and a confocal optic. During a scanning process an auxiliary focus is generated by means of at least one second radiation source and a second optic. Radiation generated by the first radiation source is collimated by a first optic and radiation generated by the second radiation source is collimated by a second optic. A retroreflection from the auxiliary focus is detected by at least one detector and is used to measuring the position of an interface and, thus, for indirectly positioning the measuring volume. The position of the auxiliary focus relative to the measuring volume is adjustable in a defined manner.

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Patent Owner(s)

Patent OwnerAddress
EVOTEC BIOSYSTEMS AG22525 HAMBURG

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Axhausen, Peter Destedt, DE 1 21
Guenther, Rolf Hamburg, DE 19 1132
Henco, Karsten Dusseldorf, DE 42 764
Mueller, Juergen Rolf Hamburg, DE 1 21
Turner, Rodney San Francisco, CA 2 55

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