Process for producing hydroxynitrile lyases
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United States of America Patent
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Mar 27, 2007
Grant Date -
Aug 7, 2003
app pub date -
Dec 27, 2000
filing date -
Dec 28, 1999
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention relates to an improved process for producing hydroxynitrile lyases. A process is provided whereby hydroxynitrile lyases may be produced by cultivation in bacteria or suitable host cells. Cells containing a gene encoding for a hydroxynitrile lyase may be precultivated, induced with a low concentration of IPTG, cultured, and lysed. The process allows for the production of relatively large quantities of hydroxynitrile lyases in a dissolved, native, and active form, and without the presence of substantial amounts of inclusion bodies, and thus allowing for the production of the lyases without requiring expensive and time consuming renaturation steps.
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