Method for exposing peptides and polypeptides on the cell surface of bacteria
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United States of America Patent
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Mar 6, 2007
Grant Date -
Jun 3, 2004
app pub date -
Oct 25, 2001
filing date -
Oct 26, 2000
priority date (Note) -
Expired
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Abstract
The inventive method allows peptides or polypeptides to be exposed on the surface of gram-negative host bacteria using specific intimin-based anchor modules. Intimins with shortened carboxy terminals have been found to be particularly suitable anchor modules for passenger domains in the exterior E. coli cell membrane. According to the method, host bacteria are transformed using vectors, on which are located a fused nucleic acid sequence consisting of a sequence segment which codes for an intimin with a shortened carboxy terminal and a nucleic acid sequence segment which codes for the passenger peptide that is to be exposed. The invention permits a particularly large number of passenger domains to be exposed on the cell surface of the bacteria, without adversely affecting the viability of the bacteria.
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