Protein production
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United States of America Patent
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Feb 27, 2007
Grant Date -
Mar 9, 2006
app pub date -
Aug 14, 2003
filing date -
Sep 18, 2002
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Proteins may be made by genetically engineered microorganisms, the protein being stored in the form of inclusion bodies (IB). The proteins in the inclusion bodies are in an insoluble and inactive form. They may be dissolved using a solubilization reagent (18), and the resulting solution diluted so that the proteins refold into the active form. This refolding of the protein is enhanced by subjecting a solution or suspension of the protein to low intensity sound waves (25), at a low enough intensity that the protein is not denatured. The intensity may be between 10 and 100 mW/cm.sup.2.
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