Methods for detection of a target nucleic acid using multi-subunit probes
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United States of America Patent
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Feb 27, 2007
Grant Date -
Aug 7, 2003
app pub date -
Jul 17, 2002
filing date -
Jul 17, 2001
priority date (Note) -
Expired
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Abstract
The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising forming a complex by incubating a sample comprising a target nucleic acid sequence with a probe comprising a first and second subunit, and/or an upstream primer, and binding the probe to the target nucleic acid such that the first and second subunits dissociate to release the first subunit and generate a signal. In certain embodiments, the upstream primer is extended with a nucleic acid polymerase to displace at least a portion of the first subunit of the probe from the target nucleic acid strand and dissociate the first and second subunits to release the first subunit of the probe and generate a signal.
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