Integrated portable biological detection system
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United States of America Patent
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Feb 6, 2007
Grant Date -
Oct 24, 2002
app pub date -
Jun 5, 2002
filing date -
Jul 7, 1994
priority date (Note) -
Expired
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Abstract
We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a 'laboratory-on-a-chip' system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
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