Apparatus and separation media for separating particles in free-flow electrophoresis
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United States of America Patent
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Jan 30, 2007
Grant Date -
Mar 18, 2004
app pub date -
Jan 17, 2003
filing date -
Jan 21, 2002
priority date (Note) -
Expired
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Abstract
A free-flow electrophoresis (FFE) apparatus and separation medium for separating particles therefore, includes a separation chamber with a dosage pump for conveying medium, electrodes for applying an electric field in the medium and sample injection points for adding a mixture of particles to be separated and fractionation points for removing the particles. Definable charge labels couple with the particles to form charge-modified particles which display with a different migratory behavior in FFE The apparatus includes focusing buffers between the separation medium and at least one electrode which has much higher electrical conductivity than the separation medium.
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