Method for separating particles in free flow electrophoresis

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 7169275
APP PUB NO 20040050697A1
SERIAL NO

10346715

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Abstract

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A method for separating particles in free-flow electrophoresis FFE includes conveying a separation medium using a dosage pump into a separation chamber; releasing the separation medium via outlets; applying an electrical field in the separation medium; coupling a particle to be separated with selectively defined charge labels for generating charge-modified particles which due to their selectively modified net surface charge display a different migratory behavior in FFE than non-charge-modified particles; adding a mixture of particles to be separated to the separation medium in the separation chamber by way of sample injection points; removing the particles separated using the separation medium by way of fractionation points; and collecting the fractions with the separated particles. Focusing buffers are created using a buffer medium between the separation medium and at least one electrode, whereby this buffer medium is much more electrically conductive than the separation medium and is fed into these focusing buffers.

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Patent Owner(s)

Patent OwnerAddress
TECAN TRADING AGSEESTRASSE 103 MANNEDORF CH-8708

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Eckerskorn, Christoph Ottobrunn, DE 3 32
Grill, Hans-Jo Mannedorf, CH 2 5
Weber, Gerhard Kirchheim, DE 71 1177
Weber, Peter Munchen, DE 122 1371
Yost, David Poolesville, MD 10 127

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