Recombinant template used for producing a carboxy-terminal modified protien and a method of producing a carboxy-terminal modified protein
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United States of America Patent
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Dec 19, 2006
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N/A
app pub date -
Jun 6, 2003
filing date -
Dec 7, 2000
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Abstract
A translation template comprising an ORF region coding for a protein, a 5' untranslated region comprising a transcription promoter and a translation enhancer and locating on the 5' side of the ORF region, and a 3' end region comprising a poly-A sequence and locating on the 3' side of the ORF region, is expressed in a translation system in the presence of an agent for modifying a C-terminal of a protein, which comprises an acceptor portion having a group capable of binding to a protein through a transpeptidation reaction in a protein translation system and a modifying portion comprising a nonradioactive modifying substance linked to the acceptor portion via a nucleotide linker, to cause protein synthesis and the synthesized protein is purified. Thus, the yield of modified protein in a method of modifying C-terminal of protein is improved and detection of protein interaction based on various intermolecular interaction detection methods is realized at an improved level.
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