System for production of helper dependent adenovirus vectors based on use of endonucleases
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United States of America Patent
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Nov 14, 2006
Grant Date -
Dec 11, 2003
app pub date -
Jan 31, 2003
filing date -
Jun 24, 1993
priority date (Note) -
Expired
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Abstract
The present invention relates to methods for efficient and reliable construction of adenovirus vectors which contain and express foreign DNA and are useful for gene transfer into mammalian cells, for vaccines and for gene therapy. The invention provides for the growth and purification of adenovirus vectors (helper dependent vectors or HDVs) from which all or most of the viral genes have been removed. The vector system described herein is a new method designed to eliminate helper viruses from the final HDV preparation by cleavage of the helper virus DNA with an endonuclease, alone or in combination with other methods known to limit the level of helper virus contamination of helper dependent vector preparations. The disclosed methods and compositions also provide for regulated control of gene expression.
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