Multiplex method of detecting sequence-specific DNA binding proteins using detection duplexes comprising unmodified nucleic acid sequences as capture tags
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United States of America Patent
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Oct 17, 2006
Grant Date -
Nov 20, 2003
app pub date -
Mar 28, 2003
filing date -
Mar 28, 2002
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Expired
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Abstract
Compositions and methods are provided for detecting and measuring DNA-binding proteins. The compositions and methods permit the simultaneous or near-simultaneous detection of multiple DNA-binding proteins in a multiplex or array format, and can be used to generate profiles of DNA binding activity by proteins, specifically, transcription factors. Multiple protein-DNA binding events in a single sample may be detected and quantitated in a high-throughput format.
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