Nucleic acid isolated from Tetrahymena which codes for a triterpenoid cyclase, its production, and use
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United States of America Patent
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Sep 12, 2006
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app pub date -
Jun 10, 2003
filing date -
Dec 1, 1999
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Abstract
The present invention relates to nucleic acids isolated from Tetrahymena which code for a ciliate-specific triterpenoid cyclase. The inventive nucleotide sequences and the polypeptide sequences derived therefrom demonstrate a surprisingly minimal sequence identity to known isoprenoid cyclases. The invention also relates to the use of nucleic acids for the regulation of triterpenoid cyclase expression in a host organism, as well as the targeted knockout or repriming of the triterpenoid cyclase gene. As a result of the altered expression of the triterpenoid cyclase, it is possible to modify and enrich the levels of multiple unsaturated fatty acids in the host organism.
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