Use of mixed duplex oligonucleotides to effect localized genetic changes in plants
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United States of America Patent
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Aug 22, 2006
Grant Date -
Oct 16, 2003
app pub date -
Aug 5, 1998
filing date -
Aug 5, 1997
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Abstract
The invention concerns the use of duplex oligonucleotides about 25 to 30 base pairs to introduce site specific genetic alterations in plant cells. The oligonucleotides can be delivered by mechanical (biolistic) systems or by electrpoporation of plant protoplasts. Thereafter plants having the genetic alteration can be generated from the altered cells. In specific embodiments the invention concerns alteration in the gene that encode acid invertase, UDP-glucose pyrophosphorylase, polyphenol oxidase, O-methyl transferase, cinnamyl alcohol dehydrogenase, ACC synthase and ACC oxidase or etr-1 or a homolog of etr-1, and plants having isolated point mutations in such genes.
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