Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
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United States of America Patent
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Aug 1, 2006
Grant Date -
Jun 12, 2003
app pub date -
Apr 19, 2002
filing date -
Apr 19, 2001
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Abstract
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.
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