Method for purifying enzymes from clostridium histolyticum using multi-stage chromatography
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United States of America Patent
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Aug 1, 2006
Grant Date -
Jul 15, 2004
app pub date -
Jun 1, 2002
filing date -
Jul 2, 2001
priority date (Note) -
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Abstract
The invention relates to a method for purifying at least one enzyme obtained in an excess fermentation of Clostridium histolyticum. It is provided for that the enzymes of the excess fermentation are separated by a multistage chromatography method by exclusively using chromatography materials on styrene/divinyl-benzene base and/or on base of in particular ceramic hydroxylapatite.
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