General means of labeling protein by using wheat embryo cell-free protein synthesis system
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United States of America Patent
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Jul 11, 2006
Grant Date -
Aug 28, 2003
app pub date -
Jul 18, 2001
filing date -
Jul 21, 2000
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Utilizing a what embryo cell-free protein synthesis system, there are provided a process for the production of selenomethionine-labeled protein, characterized in that, methionine in a wheat embryo extract for a cell-free protein synthesis obtained by a complete removal of endosperm contaminated is changed to selenomethionine and a cell-free protein synthesis is carried out using a reaction solution composition for protein synthesis containing selenomethionine instead of methionine under a batch condition or a dialysis condition and also the said protein produced as such. There are further provided a process for the production of heavy hydrogen-labeled protein using the same means and also the said protein produced as such.
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