Methods for preparing yeast with improved biotin productivity using integrating plasmids encoding biotin synthase
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United States of America Patent
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Apr 25, 2006
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app pub date -
Jan 2, 2001
filing date -
Oct 7, 2000
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Abstract
Methods for preparing yeast with improved biotin productivity using integrating plasmids encoding biotin synthase. The yeast is transformed by an integrating plasmid, which includes a Candida utilis biotin synthase gene BIO2, an assistant DNA sequence to promote integration of the plasmid into the C. utilis genome, a promoter sequence, and a selection marker. Other embodiments include Saccharomyces cerevisiae integrating plasmids.
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