Enzymatic substrate, synthesis method and uses
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United States of America Patent
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Apr 18, 2006
Grant Date -
N/A
app pub date -
Oct 25, 2000
filing date -
Oct 28, 1999
priority date (Note) -
Expired
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Abstract
The invention concerns novel enzymatic substrates of general formula (I) wherein: X represents a nitrogen atom or a carbon atom substituted with a phenyl group, said phenyl group being optionally substituted in meta or para position: Y and Z represent a hydrogen atom when X represents a carbon atom or Y and Z represent together an ether, thioether or amine bond optionally substituted with an alkyl or aryl group; R1 and R2 independently represent each H, Cl, F, I, Br and can be identical or different or R1 and R2 together represent a substituted or non-substituted fused benzene ring, R3 and R4 independently represent each H, Cl, F, I, Br and can be identical or different; R represents a group such that the O—R bond is capable of being hydrolysed by an enzyme. The invention also concerns a method for synthesizing said substrates, a composition, a kit and a method for detecting at least one micro-organism using said enzymatic substrate.
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