Detection of ligand-antiligand complex formation by electromagnetically detectable bulk property measurement
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United States of America Patent
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Mar 14, 2006
Grant Date -
Jan 22, 2004
app pub date -
Jul 17, 2002
filing date -
Jul 17, 2002
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Expired
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Abstract
Disclosed are methods for analyzing molecular binding events in which the formation of ligand/antiligand complexes can be directly detected in a mixture without requiring separation of the components of the mixture from each other by measuring bulk properties of the mixture (i.e., properties that have contributions from several or all of the components present in the mixture). Using these techniques, it is possible to screen libraries without labeling either the target antiligand or ligand. The invention also provides a method for determining the strength of ligand/antiligand binding by further analysis of the same signals.
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