HEK293 cell line for activity studies and HTS system of α1G T-type Ca2+ channel
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United States of America Patent
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Jan 10, 2006
Grant Date -
Jun 16, 2005
app pub date -
May 28, 2004
filing date -
Dec 12, 2003
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Abstract
There is provided a cell line that is prepared by transforming HEK293 cell with a human Kir2.1 gene using a retrovirus expression system, wherein the HEK293 expresses a stable α1G T-type calcium channel. The cell line responds sensitively to KCl and forms an appropriate level of the membrane voltage so that the cell signaling pathway may be investigated by the molecular biological and biochemical studies.
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