Production of motif-specific and context-independent antibodies using peptide libraries as antigens
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United States of America Patent
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Jan 3, 2006
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app pub date -
Mar 24, 2000
filing date -
Sep 4, 1998
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Abstract
A class of motif-specific, context-independent antibodies that bind conserved signal transduction motifs, such as kinase consensus substrate motifs and protein-protein binding motifs, containing one or more modified amino acids, such as a phosphorylated amino acid, is provided. The antibodies bind a plurality of peptides or proteins that contain the modified motif. Context-independent antibodies specific for single modified residues, such as phosphothreonine, are also provided. Methods for producing and using such antibodies are provided.
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