Locked nucleic acid hybrids and methods of use
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United States of America Patent
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Dec 20, 2005
Grant Date -
Jul 18, 2002
app pub date -
Apr 21, 2000
filing date -
Apr 21, 1999
priority date (Note) -
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Abstract
The invention relates to methods for inhibiting, cloning, modifying or labelling an endogenous DNA sequence using compositions comprising recombinases in combination with exogenous polynucleotides containing 'anchoring' or 'locking' sequences. The anchoring sequences serve to stabilize structures formed by the exogenous polynucleotides and the endogenous DNA. The stabilized structure thus can either serve to regulate gene transcription or replication, or can allow the endogenous sequences to be labelled or pulled out, i.e. cloned, or modified.
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