Gene coding for cyclodextrin glucanotransferase chiefly producing .gamma.-cyclodextrin and use thereof
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United States of America Patent
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Nov 1, 2005
Grant Date -
Jun 19, 2003
app pub date -
Jul 3, 2002
filing date -
Jul 11, 2001
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Abstract
The invention provides a gene (isolated nucleic acid molecule) encoding the cyclodextrin glucanotransferase which produces a considerable amount of .gamma.-cyclodextrin (.gamma.-CD) from the substrates selected from among starch and starch decomposition products such as dextrin, amylopectin and amylose; recombinant plasmids comprising this gene; transformants transformed with the recombinant plasmid; methods of manufacturing the cyclodextrin glucanotransferase by employing these transformants to act upon the substrates selected from among starch and decomposition products thereof and causing the production of .gamma.-CD as a main product; and methods of manufacturing .gamma.-CD and CD-comprising compositions having a desired CD balance (.alpha.-, .beta.- and .gamma.-CD balance) employing this recombinant cyclodextrin glucanotransferase.
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