Dye compositions which provide enhanced differential fluorescence and light scatter characteristics
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United States of America Patent
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Oct 18, 2005
Grant Date -
Sep 23, 2004
app pub date -
Mar 20, 2003
filing date -
Mar 20, 2003
priority date (Note) -
Expired
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Abstract
A composition for enhancing differential staining of RNA, DNA and granules in a sample comprising cells contains a first fluorescent dye that can bind specific binding sites and non-specific binding sites in the sample. This first dye emits fluorescence at a first wavelength. The composition contains at least an additional component, which is a second non-intercalating dye in the composition that competes with said first dye for binding to the nonspecific binding sites, or a permeabilizing agent to enhance permabilization of the dyes into the cells, or both. The molar ratio of the second dye and the first dye is at least about 20:1.
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