Method for excystation of sporocysts
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United States of America Patent
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Sep 27, 2005
Grant Date -
Jun 5, 2003
app pub date -
Oct 15, 2002
filing date -
Oct 15, 2001
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Abstract
A process to excystate protozoal sporocysts involves treatment of an infected tissue sample with a sodium hypochlorite solution to stimulate excystation of the sporocysts from the tissue. Thereafter, removal of the sodium hypochlorite solution and treatment of the sample with a cell culture media as the excystation fluid eliminates incubation and subsequent washing steps using expensive reagents. The excystation fluid contains substantially no chelating agents, proteins, enzymes or bile acids.
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