Methods and compositions for effecting homologous recombination
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United States of America Patent
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Aug 30, 2005
Issued Date -
N/A
app pub date -
Jan 18, 2002
filing date -
May 3, 2000
priority date (Note) -
Expired
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Abstract
This invention concerns a method for effecting directed mutagenesis based on a guided homologous recombination system that comprises (i) a triple helix forming oligonucleotide, (ii) a donor nucleic acid segment, and (iii) an adapter segment comprising an oligonucleotide sequence able to bind at least a portion of said donor nucleic acid through Watson-Crick base pairing.
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