Animal component free meningococcal polysaccharide fermentation and seedbank development
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United States of America Patent
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Aug 23, 2005
Grant Date -
Nov 18, 2004
app pub date -
May 16, 2003
filing date -
May 16, 2003
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Abstract
Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times the level observed with batch fermentation. This process is used successfully to produce polysaccharides of N. meningitidis serotypes, A, C, Y and W-135 and is run reproducibly at the 20 L scale and can be scaled to 400 L or more.
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