Method of characterizing fluorescent molecules or other particles using generating functions

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United States of America Patent

PATENT NO 6927401
SERIAL NO

09869581

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A method for characterizing fluorescent molecules or other particles in samples comprising the steps of: a) monitoring fluctuating intensity of fluorescence emitted by the molecules or other particles in at least one measurement volume of a non-uniform spatial brightness profile by measuring numbers of photon counts in primary time intervals by a single or more photon detectors, b) determining at least one distribution of numbers of photon counts, {circumflex over (P)}(n), from the measured numbers of photon counts, c) determining physical quantities characteristic to said particles by fitting the distribution of numbers of photon counts {circumflex over (P)}(n), wherein the fitting procedure involves calculation of a theoretical distribution function of the number of photon counts P(n) through its generating function, defined as ##EQU1##

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Patent OwnerAddress
OLYMPUS CORPORATION2951 ISHIKAWA-MACHI HACHIOJI-SHI TOKYO 192-8507

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Palo, Kaupo Haabneeme, EE 19 151

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