Cyclodextrin glucanotransferase and its method of manufacture
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United States of America Patent
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Aug 2, 2005
Grant Date -
Oct 16, 2003
app pub date -
May 23, 2001
filing date -
May 23, 2000
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Abstract
CGTase enzymatically acts on starch, dextrin, amylopectin, and amylose to produce primarily γ-CD, with quantities of β- and α-CD produced being smaller than the quantity of γ-CD produced, has an optimum pH of 10.5-11.0, an optimum temperature of about 60° C., a stable pH of 6-11, and temperature stability, exhibits residual activity of not less than 90 percent with a 15 minute-treatment at 50° C., is produced by culturing a Bacillus clarkii (for example, FERM B-7156), reacts with starch or the like to produce principally γ-cyclodextrin.
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