Methods for direct detection of individual methotrexate metabolites
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United States of America Patent
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Jul 26, 2005
Grant Date -
Sep 9, 2004
app pub date -
Mar 7, 2003
filing date -
Mar 7, 2003
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Abstract
The present invention provides a method for determining a level of a methotrexate polyglutamate (MTXPG) in a cellular extract by resolving at least one MTXPG in a cellular extract obtained from a human undergoing methotrexate therapy; and detecting at least one resolved MTXPG, thereby determining a level of the resolved MTXPG. A method of the invention can be useful, for example, for determining a level of MTXPG3, MTXPG4 or MTXPG5 or for determining a level of each methotrexate polyglutamate species (MTXPG1 to MTXPG7) present in the cellular extract.
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