Hybridization rate enhancement for substrate-bound specific nucleic acid-binding agents
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United States of America Patent
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Jul 19, 2005
Grant Date -
Sep 25, 2003
app pub date -
Mar 22, 2002
filing date -
Mar 22, 2002
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Expired
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Abstract
The invention relates to kits and methods for hybridizing nucleic acids with a specific nucleic acid-binding agent, such as a complementary nucleic acid. Previously, others have hybridized a nucleic acid with such an agent bound to a substrate. The improved methods described herein comprise binding a polycationizable attractor compound to the substrate, in addition to the agent. Examples of suitable polycationizable attractor compounds include polypeptides, including those with tunable cationizable amino acid residues, such as histidine. Compositions, kits, devices, and methods that make use of this hybridization rate enhancement technology are disclosed.
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