Highly productive packaging lines
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United States of America Patent
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Mar 29, 2005
Issued Date -
N/A
app pub date -
Oct 15, 2001
filing date -
Jul 16, 1996
priority date (Note) -
Expired
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Abstract
The invention concerns a packaging eukaryotic cell for the production of defective infections viruses carrying a transgene, characterized in that it is deficient in one cell function essential to its growth, in particular in the presence of a selection culture medium, the function being capable of being restored by the expression of an exogenous sequence introduced in the cell: either with a vector carrying transcomplementing functions of packaging cells; or with a vector carrying a transgene, and enabling the selection in a selective medium of cells carrying the said exogenous sequence.
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