Production method of recombinant rotavirus structural proteins and vaccine composition
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United States of America Patent
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Mar 15, 2005
Grant Date -
Sep 18, 2003
app pub date -
Sep 30, 2002
filing date -
Feb 12, 2000
priority date (Note) -
Expired
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Abstract
The present invention relates to a method for producing recombinant rotavirus structural proteins by culturing a transformed plant cell, and to a vaccine composition comprising the same as an effective component. More specifically, the method comprises the steps of preparing the expression vector comprising cDNA fragments encoding rotavirus structural proteins; transforming a plant cell with the expression vector; and recovering the vaccine composition, including rotavirus antigens, from the cell culture. The method for producing the recombinant rotavirus structural proteins is advantageous in that higher yields of more than 0.3 mg/L are obtained, as well as a lower cost and a lower contamination hazard than those of eukaryotic expression systems such as baculovirus and animal cell. Furthermore, the method can be used to produce a edible vaccine. The invention also proves that the rotavirus structural protein can be produced with a high aspect rotary vessel reactor.
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