High level secretory expression system of intact MK family protein
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United States of America Patent
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Mar 15, 2005
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N/A
app pub date -
Apr 30, 2001
filing date -
Aug 10, 1998
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Abstract
Under the regulation of a methanol-inducible alcohol oxidase gene (AOX1) promoter and a transcription termination sequence originating from Pichia pastoris, Pichia yeast, which has been transformed with an expression vector containing an MK family protein gene ligated to an a1 factor signal sequence originating from Saccharomyces cerevisiae, is cultured and an intact MK family protein is thus expressed and secreted in a large amount into the culture supernatant.
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