Measurement of fluorescence using capillary isoelectric focusing
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Feb 8, 2005
Grant Date -
Dec 27, 2001
app pub date -
Apr 12, 2001
filing date -
Apr 13, 2000
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
Improved apparatus is disclosed for carrying out axially illuminated laser induced fluorescence whole-column imaging detection in the capillary isoelectric focusing of proteins. The separation capillary was made of low refractive index Teflon conditioned with methylcellulose to reduce electroosmotic flow and a small amount of high refractive index organic solvent (glycerol) was added to the sample mixture. It was found that an axially directed laser excitation beam was propagated essentially with total internal reflection, so that minimum interference arose from stray light or from scattering light originating from the wall of the capillary. With the naturally fluorescent protein R-phycoerythrin, a concentration detection limit LOD 10.sup.-11 M or mass LOD 10.sup.-17 Mo was obtained.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully