Methods and compositions for extracting proteins from cells
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United States of America Patent
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Nov 23, 2004
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app pub date -
Jan 18, 2002
filing date -
Jan 19, 2001
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Abstract
The present invention relates to a process of releasing a protein, recombinant or otherwise, from a cell. The process of the present invention involves contacting a host cell containing a protein of interest with a solution comprising one or more detergents and one or more reducing agents. The methods of the invention are particularly suitable to large scale production of recombinant products.
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