Method for increasing survival rate of cells in animal cell culture under hypoxia condition
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United States of America Patent
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Nov 16, 2004
Grant Date -
Jan 30, 2003
app pub date -
Jul 12, 2002
filing date -
Jan 12, 2000
priority date (Note) -
Expired
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Abstract
The present invention relates to a method for increasing survival rate of cells in animal cell culture under hypoxia condition by adding antibiotics to the culture media. The method of present invention comprises a step of culturing animal cells in culture media containing antibacterial agent of quinolones, quinones, aminoglycosides or chloramphenicol at the concentration range of 0.1 to 1000 .mu.g/ml. The invented method can be practically applied for high-density animal cell culture to produce recombinant proteins or cultured cells.
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