Fluorescence polarization-based homogeneous assay for deoxynivalenol determination in grains
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United States of America Patent
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Nov 2, 2004
Grant Date -
May 9, 2002
app pub date -
Jul 11, 2001
filing date -
Sep 11, 2000
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Abstract
A homogeneous assay for determining the deoxynivalenol (DON) content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with water. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to DON. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating DON to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The DON concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of DON solutions of known concentration.
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