Fluorescent pH indicators for intracellular assays
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United States of America Patent
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Oct 5, 2004
Grant Date -
Apr 10, 2003
app pub date -
Mar 27, 2002
filing date -
Aug 2, 2001
priority date (Note) -
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Abstract
Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2',7'-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.
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