Method for synthesis of polypeptides in cell-free systems

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United States of America Patent

PATENT NO 6783957
SERIAL NO

09937474

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The method of polypeptide synthesis in eukaryotic or porkaryotic cell-free systems based on a modified verion of synthesis in the continuous flow or continuous exchange modes when, in addition to input into the reaction mixture of components maintaining the synthesis and removal from the reaction mixture of low molecular weight components inhibiting the synthesis, the concentration of at least one of the components selected from the group consisting of Mg.sup.2+, K.sup.+, NTP, polyamines or their combinations determining the productivity of the synthesis is continuously changed within the given range of concentrations, while the concentrations of the other components are maintained constant.

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Patent Owner(s)

Patent OwnerAddress
ROCHE DIAGNOSTICS GMBHSANDHOFER STRASSE 116 68305 MANNHEIM 68305
INSTITUTE OF PROTEIN RESEARCHRUSSIAN ACADEMY OF SCIENCES INSTITUTSKAYA ST 4 PUSHCHINO 142290

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Biryukov, Sergey Vladimirovich Moscow Region, RU 4 26
Shirokov, Vladimir Anatolievich Moscow Region, RU 3 14
Simonenko, Peter Nikolaevich late of Moscow Region, RU 1 7
Spirin, Alexander Sergeyevich Moscow Region, RU 3 14

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