Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
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United States of America Patent
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Aug 3, 2004
Grant Date -
Aug 16, 2001
app pub date -
Feb 15, 2001
filing date -
Aug 13, 1998
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Abstract
The present invention relates to methods and apparatuses for analyzing molecules, particularly polymers, and molecular complexes with extended or rod-like conformations. In particular, the methods and apparatuses are used to identify repetitive information in molecules or molecular ensembles, which is interpreted using an autocorrelation function in order to determine structural information about the molecules. The methods and apparatuses of the invention are used for, inter alia, determining the sequence of a nucleic acid, determining the degree of identity of two polymers, determining the spatial separation of specific sites within a polymer, determining the length of a polymer, and determining the velocity with which a molecule penetrates a biological membrane.
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