Detection of clonal T-cell receptor-.gamma. gene rearrangement by PCR/temporal temperature gradient gel electrophoresis (TTGE)
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United States of America Patent
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Apr 13, 2004
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N/A
app pub date -
Jun 21, 2001
filing date -
Jun 21, 2001
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Expired
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Abstract
The present invention provides methods and compositions for detecting and analyzing clonal T-cell receptor (TCR) gene rearrangement using temporal temperature gradient gel electrophoresis (TTGE), which employs a gradual and uniform increase in the temperature of a constant denaturing gel to resolve different DNA molecules based on base pair composition. The present invention also provides methods and compositions for providing appropriate DNA migration markers for TTGE analysis.
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