Artificial antibody polypeptides
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United States of America Patent
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Jan 6, 2004
Grant Date -
Feb 14, 2002
app pub date -
Jun 12, 1998
filing date -
Jun 12, 1997
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Abstract
A fibronectin type III (Fn3) polypeptide monobody, a nucleic acid molecule encoding said monobody, and a variegated nucleic acid library encoding said monobody, are provided by the invention. Also provided are methods of preparing a Fn3 polypeptide monobody, and kits to perform said methods. Further provided is a method of identifying the amino acid sequence of a polypeptide molecule capable of binding to a specific binding partner (SBP) so as to form a polypeptide:SSP complex, and a method of identifying the amino acid sequence of a polypeptide molecule capable of catalyzing a chemical reaction with a catalyzed rate constant, k.sub.cat, and an uncatalyzed rate constant, k.sub.uncat, such that the ratio of k.sub.cat /k.sub.uncat is greater than 10.
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