Transfusion medicine leukodepletion filter devices as a source of genetic material for genotyping studies
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United States of America Patent
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Dec 30, 2003
Grant Date -
Jul 11, 2002
app pub date -
Aug 15, 2001
filing date -
Aug 16, 2000
priority date (Note) -
Expired
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Abstract
The present invention provide methods for utilizing spent leukodepletion filter devices as a source of material for the isolation and analysis of genomic DNA (gDNA), including polymorphism, genotyping, and pharmacogenomic studies. Cellular retentate with the filter contains leukocytes, which are lysed to release the nuclei. The nuclei are lysed or ruptured to release genomic DNA, which is then isolated and used for subsequent analysis.
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