PROCEDURE FOR THE ANALYSIS OF THE FUNCTIONAL ACTIVATION OF LEUKOCYTES, PLATELETS AND OTHER CELLS, INDUCED IN VIVO OR IN VITRO, BASED ON THE STABILIZATION OF CYTOPLASMIC MEMBRANE PROTEINS AND ITS DETECTION USING QUANTITATIVE CYTOMETRIC METHODS IN THE ABSENCE OF ADDITIONAL SAMPLE MANIPULATION
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United States of America Patent
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Dec 23, 2003
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app pub date -
May 30, 2000
filing date -
May 31, 1999
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Abstract
Procedure for the study of the functional activation of leukocytes, platelets and other cells, produced in vivo or induced in vitro, based on the stabilization of cytoplasmic membrane proteins and its detection using quantitative cytometric methods in the absence of any further manipulation of the sample. The procedure includes the sequential incubation of the sample with: 1) either one or a mixture of more than one protease specific inhibitors and, 2) a combination of several fluorochrome-conjugated monoclonal antibodies; to analyze the expression of surface proteins using immunofluorescence methods and quantitative cytometry.
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