Method of increasing recovery of heterologous active enzymes produced in plants
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United States of America Patent
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Oct 14, 2003
Grant Date -
Apr 4, 2002
app pub date -
Jun 14, 2001
filing date -
Jun 15, 2000
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Abstract
A method of increasing recovery of active enzyme produced in a plant is provided where the enzyme requires a transitional metal cofactor for activation. The metal cofactor is supplied to the enzyme during plant development, during extraction, or after extraction. Recovery of active enzyme is also provided by incubating the extracted enzyme at a non-enzyme degrading temperature. Addition of a negative ion salt further improves active enzyme recovery. Optimum salt concentrations for recovery of laccase from plants using copper solutions is provided.
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