Enhanced catalyzed reporter deposition

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 6593100
APP PUB NO 20010039035A1
SERIAL NO

09859988

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent comprising an inorganic salt, an organic compound having the formula ##STR1## wherein when X is B(OH).sub.2, Y is I, or wherein when X is OH, Y is a halogen, or Q-R wherein Q is a linear or branched 1-12 heteroatom alkyl wherein tie heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from --OH, --COOH, --NH.sub.2, and --SH, and wherein R is selected from --OH, --COOH, --NH.sub.2, and --CH.sub.3 ; or mixtures of the inorganic salt and the organic compound. A compound having the formula: ##STR2## wherein R.sup.1 is --CH.sub.2 OH, --COOH, and n is 1-8.

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Patent Owner(s)

Patent OwnerAddress
WASHINGTON UNIVERSITYONE BROOKINGS DRIVE ST LOUIS MO 63130
MEN LIFE SCIENCE PRODUCTS INC549 ALBANY STREET BOSTON MA 02118

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Adler, Karl Edwin Newburyport, MA 9 46
Bobrow, Mark Norman Lexington, MA 19 476
Roth, Kevin Aaron Webster Groves, MO 4 26

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