Methods and compositions for synthesis of long chain polyunsaturated fatty acids
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United States of America Patent
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Jul 8, 2003
Issued Date -
N/A
app pub date -
Aug 19, 1999
filing date -
Apr 11, 1997
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Expired
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Abstract
The present invention relates to a fatty acid .DELTA.5-desaturase able to catalyze the conversion of dihomo-gamma-linolenic acid to arachidonic acid. Nucleic acid sequences encoding a .DELTA.5-desaturase, nucleic acid sequences which hybridize thereto, DNA constructs comprising a .DELTA.5-desaturase gene, and recombinant host microorganism or animal expressing increased levels of a .DELTA.5-desaturase are described. Methods for desaturating a fatty acid at the .DELTA.5 position and for producing arachidonic acid by expressing increased levels of a .DELTA.5 desaturase are disclosed. Fatty acids, and oils containing them, which have been desaturated by a .DELTA.5-desaturase produced by recombinant host microorganisms or animals are provided. Pharmaceutical compositions, infant formulas or dietary supplements containing fatty acids which have been desaturated by a .DELTA.5-desaturase produced by a recombinant host microorganism or animal also are described.
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