Gene sequencer and method for determining the nucleotide sequence of a chromosome
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United States of America Patent
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May 20, 2003
Grant Date -
Apr 18, 2002
app pub date -
May 21, 2001
filing date -
Feb 21, 1997
priority date (Note) -
Expired
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Abstract
A method for determining the nucleotide sequence of a chromosome. The chromosome is hydrolyzed to form a mixture of oligonucleotides having unknown nucleotide sequences. The mixture is exposed to a library of oligonucleotide probe pairs having known sequences. The probe pairs are located so that they hybridize to outer opposed sections of the chromosomal oligonucleotides. The use of oligonucleotide probe pairs provides for sequencing of relatively long unknown DNA sequences using relatively short oligonucleotide probes.
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