Bacteriophage assay
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United States of America Patent
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Apr 29, 2003
Grant Date -
N/A
app pub date -
Jan 12, 2001
filing date -
May 2, 1998
priority date (Note) -
Expired
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Abstract
There is provided an assay suitable for the typing of bacterial strains. In the assay a predetermined amount of phage is combined with a bacterial isolate of unknown strain, the mixture being located in a suitable container. The mixture of phage and bacteria is conveniently held in a liquid or semi-liquid medium facilitating interaction of the two species. The extent of bacterial growth in the presence of the phage is measured by conventional means, preferably by means of an OD reading. Desirably the phage is retained in the selected container, which is conveniently a micro-titer plate, through use of a fixant such as 5% gelatin.
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